Physical Mapping

In vitro Reconstruction of Aspergillus nidulans Genome

A physical map of the 31-megabase Aspergillus nidulans genome is reported, in which 94% of 5,134 cosmids are assigned to 49 contiguous segments. The physical map is the result of a two-way ordering process, in which clones and probes were ordered simultaneously on a binary DNA/DNA hybridization matrix. Compression by elimination of redundant clones resulted in a minimal map, which is a chromosome walk. Repetitive DNA is non-randomly dispersed in the A. nidulans genome, reminiscent of heterochromatic banding patterns of higher eukaryotes. We hypothesize gene clusters may arise by horizontal transfer and spread by transposition to explain the nonrandom pattern of repeats along chromosomes.


Prade R, Griffith J, Arnold J and Timberlake W 1997 In vitro reconstruction of Aspergillus nidulans genome Proceedings of the National Academy of Sciences USA 94: 14564-14569

Wang Y, Prade R, Griffith J, Timberlake W and Arnold J 1994 A new, fast random cost algorithm for physical mapping Proceedings of the National Academy of Sciences USA 91: 11094-11098


Prade R 2000 The reliability of the Aspergillus nidulans physical map Fungal Genetics and Biology 29:175-185

Xiong M, Chen H, Prade R, Wang Y, Griffith J, Timberlake WE and Arnold J 1997 On the consistency of a physical mapping method to reconstruct a chromosome in vitro Genetics 142:267-284

Wang Y, Prade R, Griffith J, Timberlake W and Arnold J 1994 ODS_BOOTSTRAP: Assessing the statistical reliability of physical maps by bootstrap resampling CABIOS 10:625-634


Cell & Molecular Biology
ONLINE  Cell & Molec. Biol.
Medical Mycology
Eukaryotic Genetics
Biomass Technology
Biomass Degradation
High Temp. Enzymes
Protein expression systems

Molecular Genetics
Gene Silencing
Stress Responses
Gene Regulation

DNA Bioinformatics
Structural Protein Modeling
Physical Mapping