Accumulation of Stress and Inducer-Dependent Plant-Cell-Wall-Degrading Enzymes During Asexual Development in Aspergillus nidulans






ARTICLE

Prade R Ayoubi P Krishnan S Macwana S and Russell H 2001 Accumulation of Stress and Cell Wall Degrading Enzyme Associated Transcripts During Asexual Development in Aspergillus nidulans Genetics 157: 957-96

Determination and interpretation of fungal gene expression profiles based on digital reconstruction of expressed sequenced tags (ESTs) are reported. A total of 51,524 DNA sequence files processed with PipeOnline resulted in 9775 single and 5660 contig unique ESTs, 31.2% of a typical fungal transcriptome. Half of the unique ESTs shared homology with genes in public databases, 35.8% of which are functionally defined and 64.2% are unclear or unknown. In Aspergillus nidulans 86% of transcripts associate with intermediate metabolism functions, mainly related to carbohydrate, amino acid, protein, and peptide biosynthesis. During asexual development, A. nidulans unexpectedly accumulates stress response and inducer-dependent transcripts in the absence of an inducer. Stress response genes in A. nidulans ESTs total 1039 transcripts, contrasting with 117 in Neurospora crassa, a 14.3-fold difference. A total of 5.6% of A. nidulans ESTs implicate inducer-dependent cell wall degradation or amino acid acquisition, 3.5-fold higher than in N. crassa. Accumulation of stress response and inducer-dependent transcripts suggests general derepression of cis-regulation during terminal asexual development. 

MORE ARTICLES ABOUT GENE REGULATION

Vanzela A P F C, Said S and Prade R (2011) Phosphatidylinositol phospholipase C mediates carbon sensing and vegetative nuclear duplication rates in Aspergillus nidulans. Canadian Journal of Microbiology 57: 611-616

Brito-Madurro A, Prade R, Madurro J, Santos M, Peres N, Cursino-SantosJ, Martinez-Rossi N and Rossi A 2008 A single amino acid substitution in one of the lipases of Aspergillus nidulans confers resistance to the antimycotic drug undecanoic acid. Biochemical Genetics 46:557-65 

Krappmann S, Jung N, Medic B, Prade R and Braus G 2006 The Aspergillus nidulans F-Box Protein GrrA Links SCF Activity to Meiosis Molecular Microbiology 61:76-88

Pócsi I, Prade R and Penninckx M 2004 Glutathione, Metabolism in Fungi Advances in Microbial Physiology 49:1-76 - Invited Publication (Peer-reviewed)

Graminha MAS, Rocha EMF, Prade RA and Martinez-Rossi NM 2004 Terbinafine resistance mediated by salicylate-1-monooxygenase in Aspergillus nidulans Antimicrobial Agents and Chemotherapy 48:3530-3535

Martinez-Rossi N, Ferreira-Nozawa M, Graminha M, Nozawa S, Fachin A, Cervelatti E, Prade R and Rossi A 2003 Molecular aspects of dermatophyte-host interactions In "Fungi in human and animal health" edited by RKS Kushwaha Scientific Publishers  - Invited Publication (Peer-reviewed)

Stiles J, Prade R and Greene C 1996 Detection of Toxoplasma gondii in feline and canine biological samples by use of the polymerase chain reaction American Journal of Veterinary Research 57:264-267

Prade R and Timberlake W 1994 The Penicillium chrysogenum and Aspergillus nidulans wetA developmental regulatory genes are functionally equivalent Molecular and General Genetics 244: 539-547

Prade R and Timberlake, W 1993 The Aspergillus nidulans brlA regulatory locus consists of overlapping transcription units that are individually required for conidiophore development EMBO J 12:2439-2447

Milagres A, Lacis L and Prade R 1993 Characterization of xylanase production by a local isolate of Penicillium janthinellum Enzyme and Microbial Technology 15:248-253


http://www.genetics.org/content/157/3/957.longhttp://www.genetics.org/content/157/3/957.longhttp://www.nrcresearchpress.com/doi/abs/10.1139/w11-034?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub%3dpubmedhttp://www.nrcresearchpress.com/doi/abs/10.1139/w11-034?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub%3dpubmedhttp://www.springerlink.com/content/773k7373760020x2/http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2958.2006.05215.x/abstract;jsessionid=11CBAE0132A3AF729D17907808CD2F86.d03t01?systemMessage=Wiley+Online+Library+will+be+disrupted+3+Mar+from+10-13+GMT+for+monthly+maintenancehttp://www.sciencedirect.com/science/article/pii/S0065291104490018http://www.sciencedirect.com/science/article/pii/S0065291104490018http://www.ncbi.nlm.nih.gov/pmc/articles/PMC514740/http://www.ncbi.nlm.nih.gov/pmc/articles/PMC514740/http://www.cabdirect.org/abstracts/20043150747.html;jsessionid=71701238019C9B7B6FF9416B46C5483Ahttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC413479/http://www.sciencedirect.com/science/article/pii/014102299390145Rshapeimage_1_link_0shapeimage_1_link_1shapeimage_1_link_2shapeimage_1_link_3shapeimage_1_link_4shapeimage_1_link_5shapeimage_1_link_6shapeimage_1_link_7shapeimage_1_link_8shapeimage_1_link_9shapeimage_1_link_10shapeimage_1_link_11shapeimage_1_link_12
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