Oklahoma State University
Gilbert H. John, Ph.D.
FAX (405) 744-6790
307 Life Science East
Stillwater, OK 74078
Study the ability of intestinal microflora to metabolize xenobiotics. Specific area of interest involves studying the molecular and biochemical aspects of cytochrome P450-like proteins in microflora.
Develop biological sensors using microbial and mammalian enzymes to specifically detect toxin compounds.
Colorado State University, Fort Collins, CO B.S. 1985 Microbiology
Colorado State University, Fort Collins, CO Ph.D. 1990 Microbiology
Special Training and Previous Position
Associate Professor, Oklahoma State University, Department of Microbiology and Molecular Genetics, Stillwater, OK, 2001-present.
Assistant Professor, Oklahoma State University, Department of Microbiology and Molecular Genetics, Stillwater, OK, 1995-2001
Assistant Research Scientist, University of Arizona, Department of Pharmacology and Toxicology, Tucson, AZ, 1992-1995
Associate Adjunct Faculty, Microbiology Instructor, Pima Community College, Tucson, AZ, 1994-1995
Postdoctoral Fellow, Centers for Disease Control, Division of Molecular Biology, Lyme Disease Section, Fort Collins, CO, 1990-1992
Teaching Adjunct Faculty, Microbiology Instructor, Front Range Community College, Fort Collins, CO, 1992
Assistant for Microbiology 200, Colorado State University, 1989
We are interested in the function of P450-like cytochromes, particularly in microbial systems. To this end, we have pursued three research areas. First, we have investigated the presence of P450-like cytochromes, an enzyme involved in the metabolism of xenobiotics, in Eubacterium, and Bifidobacterium species, using a combination of molecular biology, microbiology and biochemistry techniques. Secondly, we have investigated the interaction of intestinal microbes with various xenobiotics (surfactants, oral drugs). Lastly, the normal physiological processes associated with cytochrome P450 have been exploited in terms of developing a biological sensor for xenobioitic detection.