Functional Genomics and Evolution of Photosynthetic Systems, (2012) Volume 33 in the series Advances in Photosynthesis and Respiration, Series Editor: Govindjee, Springer, New York, Burnap, RL and WFJ Vermaas, W, Editors. 427 pages, ISBN-10: 9400715323
Miller NT, Vaughn MD, Burnap RL. Electron flow through NDH-1 complexes is the major driver of cyclic electron flow-dependent proton pumping in cyanobacteria. Biochim Biophys Acta Bioenerg. 2020:148354; doi: 10.1016/j.bbabio.2020.148354.
Chernev, S. Fischer, J. Hoffmann, N. Oliver, R. Assunção, B. Yu, R.L. Burnap, I. Zaharieva, D.J. Nürnberg, M. Haumann, H. Dau, Light-driven formation of manganese oxide by today's photosystem II supports evolutionarily ancient manganese-oxidizing photosynthesis, Nat Commun, 11 (2020) 6110.
A.P. Avramov, H.J. Hwang, R.L. Burnap, The role of Ca2+ and protein scaffolding in the formation of nature’s water oxidizing complex, Proc Natl Acad Sci U S A. 2020 Nov 10;117(45):28036-28045. doi: 10.1073
de Lichtenberg C., Avramov AP, Zhang M, Mamedov F, Burnap RL, J. Messinger J, (2020) The D1-V185N mutation alters substrate water exchange by stabilizing alternative structures of the Mn(4)Ca-cluster in photosystem II, Biochim Biophys Acta Bioenerg, (2020) 10.1016/j.bbabio.2020.148319
Kaur, D., Gisriel, C., Burnap, R., Fromme, P. and Govindjee, G., (2019). “Gordon research conference 2019: From the biophysics of natural and artificial photosynthesis to bioenergy conversion.” Current Plant Biology, p.100129.
Miller NT and Burnap RL (2018) Navigating the fitness landscape using multiallele genome editing. Commentary Proc Natl Acad Sci U S A 115(50):12547-12549. doi: 10.1073/pnas.1818285115.
Zhang CC , Zhou CZ, Burnap RL, and Peng L (2018) Carbon/nitrogen metabolic balance: lessons from cyanobacteria. (Review Article) Trends in Plant Science (in press)
Artier J, Holland SC, Miller NT, Zhang MQ, Burnap RL (2018) Synthetic DNA system for structure-function studies of the high affinity CO2 uptake NDH-13 protein complex in cyanobacteria. Biochem Biophys Acta doi: 10.1016/j.bbabio.2018.06.015
Kim CJ, Bao H, Burnap RL, Debus RJ. (2018) Impact of D1-V185 on the Water Molecules that facilitate O2 Formation by the Catalytic Mn4CaO5 Cluster in Photosystem II, Biochemistry 57(29):4299-4311. doi: 10.1021/acs.biochem.8b00630
Cano M, Holland SC, Artier J, Burnap RL, Ghirardi M, Morgan JA, and Yu, JP (2018) Glycogen synthesis and metabolite overflow contribute to energy balancing in cyanobacteria, Cell Reports 23:667-672.
Bao H, Burnap RL (2016) Photoactivation: The light-driven assembly of the water oxidation complex of photosystem II. Front Plant Sci. 7:578-91. doi: 10.3389/fpls.2016.00578
Holland SC, Artier J, Miller NT, Cano M, Yu J, Ghirardi ML, Burnap RL (2016) Impacts of genetically engineered alterations in carbon sink pathways on photosynthetic performance. Algal Research 20:87-99 http://dx.doi.org/10.1016/j.algal.2016.09.021
Zawar PA, Javalkote VS, Burnap RL, Mahulikar PP, Puranik PR (2016) CO2 capture using limestone for cultivation of the freshwater microalga Chlorella sorokiniana PAZ and the cyanobacterium Arthrospira sp. VSJ. Bioresource Technology 221: 498-509
Bao H, Burnap RL (2015) Structural rearrangements preceding dioxygen formation by the water oxidation complex of photosystem II Proc Natl Acad Sci U S A. 112(45): E6139-47
Burnap RL, Hagemann, M, Kaplan, A (2015) Regulation of the CO2 concentrating mechanism in cyanobacteria (Review Article) Life 2015, 5(1):348-71. doi: 10.3390/life5010348.
Burnap, RL (2015) Systems and Photosystems: Cellular limits of autotrophic productivity in cyanobacteria. Front Bioeng Biotechnol 10.3389/fbioe.2015.00001 3:1. doi: 10.3389/fbioe.2015.00001
Holland SC, Kappell AD, Burnap, RL (2014) Redox changes accompanying inorganic carbon limitation in Synechocystis sp. PCC 6803. Biochem Biophys Acta online:doi:10.1016/j.bbabio.2014.12.001
Nagarajan A, Burnap RL (2014) Parallel expression of alternate forms of psbA2 gene provides evidence for the existence of a targeted D1 repair mechanism in Synechocystis sp. PCC 6803. Biochem Biophys Acta 1837(9):1417-26
Service RJ, Yano J, McConnell I, Hwang HJ, Niks D, Hille R, Wydrzynski T, Burnap RL, Hillier W, Debus RJ (2013) Participation of Glutamate-333 of the D1 Polypeptide in the Ligation of the Mn4CaO5 Cluster in Photosystem II. Biochemistry 52:8452-64
Dilbeck PL, Bao H, Neveau, Burnap RL. (2013) Perturbing the water cavity surrounding the manganese cluster by mutating the residue D1-Valine 185 has a strong effect on the water oxidation mechanism of Photosystem II. Biochemistry 52:6824-33
Burnap RL, Nambudiri R, Holland S. (2013) Regulation of the carbon-concentrating mechanism in the cyanobacterium Synechocystis sp. PCC6803 in response to changing light intensity and inorganic carbon availability. Photosynth Res. 118, 115-124
Bao H, Dilbeck PL, Burnap RL. (2013) Proton transport facilitating water-oxidation: the role of second sphere ligands surrounding the catalytic metal cluster. Photosynth Res. 116:215-29
Chiu YF, Chen YH, Roncel M, Dilbeck PL, Huang JY, Ke SC, Ortega JM, Burnap RL, and Chu HA (2013) Spectroscopic and functional characterization of cyanobacterium Synechocystis PCC 6803 mutants on the cytoplasmic-side of cytochrome b559 in photosystem II Biochim Biophys Acta 1827:507-19)
Daley SME, Kappell AD, Carrick MJ, Burnap, RL (2012) Regulation of the Cyanobacterial CO2-Concentrating Mechanism Involves Internal Sensing of NADP+ and α-Ketogutarate Levels by Transcription Factor CcmR. PLoS ONE 7(7): e41286. doi:10.1371/journal.pone.0041286
Dilbeck, PL, Hwang, HJ, Zaharieva, I, Gerencser, L., Dau, H, and Burnap, RL (2012) The mutation D1-D61N in Synechocystis sp. PCC 6803 allows the observation of pH-sensitive intermediates in the formation and release of O2 from Photosystem II, Biochemistry 51:1079-91
Nagarajan A, Winter R, Eaton-Rye J, Burnap RL. (2011) A synthetic DNA and fusion PCR approach to the ectopic expression of high levels of the D1 protein of photosystem II in Synechocystis sp. PCC 6803 J Photochem Photobiol B. 104, 212-219
Service RJ, Yano J, McConnell I, Hwang HJ, Niks D, Hille R, Wydrzynski T, Burnap RL, Hillier W, Debus RJ (2010) Participation of Glutamate-354 of the CP43 Polypeptide in the Ligation of Manganese and the Binding of Substrate Water in Photosystem II, Biochemistry. 2010 50:63-81
Hung CH, Hwang HJ, Chen YH, Chiu YF, Ke SC, Burnap RL, Chu H.A. (2009) Spectroscopic and functional characterizations of cyanobacterium Synechocystis PCC 6803 mutants on and near the heme axial-ligand of cytochrome B559 in photosystem II. J Biol Chemistry 285: 5653-63
Strickler, M. A., Hwang, H. J., Burnap, R. L., Yano, J., Walker, L. M., Service, R. J., Britt, R. D., Hillier, W., and Debus, R. J. (2008) Glutamate-354 of the CP43 polypeptide interacts with the oxygen-evolving Mn4Ca cluster of photosystem II: A preliminary characterization of the Glu354Gln mutant, Philos Trans R Soc Lond B Biol Sci 363, 1179-1187.
Hwang, H. J., Nagarajan, A., McLain, A., and Burnap, R. L. (2008) Assembly and disassembly of the photosystem II manganese cluster reversibly alters the coupling of the reaction center with the light-harvesting phycobilisome, Biochemistry 47, 9747-9755.
Bentley, F. K., Luo, H., Dilbeck, P., Burnap, R. L., and Eaton-Rye, J. J. (2008) Effects of inactivating psbM and psbT on photodamage and assembly of photosystem II in Synechocystis sp. PCC 6803, Biochemistry 47, 11637-11646.
Kommalapati, M., Hwang, H. J., Wang, H. L., and Burnap, R. L. (2007) Engineered ectopic expression of the psbA gene encoding the photosystem II D1 protein in Synechocystis sp. PCC6803, Photosynth Res 92, 315-325.
Hwang, H. J., McLain, A., Debus, R. J., and Burnap, R. L. (2007) Photoassembly of the manganese cluster in mutants perturbed in the high affinity Mn-binding site of the H2O-oxidation complex of photosystem II, Biochemistry 46, 13648-13657.
Hwang, H. J., Dilbeck, P., Debus, R. J., and Burnap, R. L. (2007) Mutation of arginine 357 of the CP43 protein of photosystem II severely impairs the catalytic S-state cycle of the H2O oxidation complex, Biochemistry 46, 11987-11997.
Sakon, J. J., and Burnap, R. L. (2006) An analysis of potential photosynthetic life on Mars, International Journal of Astrobiology 5.
Hwang, H. J., and Burnap, R. L. (2005) Multiflash experiments reveal a new kinetic phase of photosystem II manganese cluster assembly in Synechocystis sp. PCC6803 in vivo, Biochemistry 44, 9766-9774.
Andrews, H., Li, Z., Altuve-Blanco, A., Rivera, M., and Burnap, R. L. (2005) Expression, mutagenesis, and characterization of recombinant low-potential cytochrome c550 of photosystem II, Biochemistry 44, 6092-6100.
Wang, H. L., Postier, B. L., and Burnap, R. L. (2004) Alterations in global patterns of gene expression in Synechocystis sp. PCC 6803 in response to inorganic carbon limitation and the inactivation of ndhR, a LysR family regulator, J Biol Chem 279, 5739-5751.
Tu, C. J., Shrager, J., Burnap, R. L., Postier, B. L., and Grossman, A. R. (2004) Consequences of a deletion in dspA on transcript accumulation in Synechocystis sp. strain PCC6803, J Bacteriol 186, 3889-3902.
Li, Z., Andrews, H., Eaton-Rye, J. J., and Burnap, R. L. (2004) In situ effects of mutations of the extrinsic cytochrome c550 of photosystem II in Synechocystis sp. PCC6803, Biochemistry 43, 14161-14170.
Burnap, R. L. (2004) D1 protein processing and Mn cluster assembly in light of the emerging photosystem II structure., Physical Chemistry & Chemical Physics 6.
Postier, B. L., Wang, H. L., Singh, A., Impson, L., Andrews, H. L., Klahn, J., Li, H., Risinger, G., Pesta, D., Deyholos, M., Galbraith, D. W., Sherman, L. A., and Burnap, R. L. (2003) The construction and use of bacterial DNA microarrays based on an optimized two-stage PCR strategy, BMC Genomics 4, 23.
Postier, B. L., and Burnap, R. L. (2003) Functional and Comparative Genomics of Cyanobacteria, in Genomics of Plants and Fungi (Prade, R. A., and Bohnert, H. J., Eds.), pp 203-235, Marcel Dekker, New York.
Wang, H. L., Postier, B. L., and Burnap, R. L. (2002) Polymerase chain reaction-based mutageneses identify key transporters belonging to multigene families involved in Na+ and pH homeostasis of Synechocystis sp. PCC 6803, Mol Microbiol 44, 1493-1506.
Wang, H. L., Postier, B. L., and Burnap, R. L. (2002) Optimization of fusion PCR for in vitro construction of gene knockout fragments, Biotechniques 33, 26-30
Li, Z., and Burnap, R. L. (2002) Mutations of basic arginine residue 334 in the D1 protein of Photosystem II lead to unusual S2 state properties in Synechocystis sp. PCC 6803, Photosynth Res 72, 191-201.
Li, Z. L., and Burnap, R. L. (2001) Mutations of arginine 64 within the putative Ca2+-binding lumenal interhelical a-b loop of the photosystem II D1 protein disrupt binding of the manganese stabilizing protein and cytochrome c(550) in Synechocystis sp. PCC6803, Biochemistry 40, 10350-10359.
Hillier, W., Hendry, G., Burnap, R. L., and Wydrzynski, T. (2001) Substrate water exchange in photosystem II depends on the peripheral proteins, J Biol Chem 276, 46917-46924.
Debus, R. J., Campbell, K. A., Gregor, W., Li, Z. L., Burnap, R. L., and Britt, R. D. (2001) Does histidine 332 of the D1 polypeptide ligate the manganese cluster in photosystem II? An electron spin echo envelope modulation study, Biochemistry 40, 3690-3699.
Bohnert, H. J., Ayoubi, P., Borchert, C., Bressan, R. A., Burnap, R. L., Cushman, J. C., Cushman, M. A., Deyholos, M., Fischer, R., Galbraith, D. W., Hasegawa, P. M., Jenks, M., Kawasaki, S., Koiwa, H., Kore-eda, S., Lee, B. H., Michalowski, C. B., Misawa, E., Nomura, M., Ozturk, N., Postier, B., Prade, R., Song, C. P., Tanaka, Y., Wang, H., and Zhu, J. K. (2001) A genomics approach towards salt stress tolerance, Plant Physiol. Biochem. 39, 295-311.
Li, Z., Bricker, T. M., and Burnap, R. (2000) Kinetic characterization of His-tagged CP47 photosystem II in Synechocystis sp. PCC6803, Biochim Biophys Acta 1460, 384-389.
Huang, A. H. H., Liu, H., and Hedrick, G. E. R. L. B. (2000) Genome-wide polymerase chain reaction primer design., in Applied Computing (Carroll, J., Damiani, E., Haddad, H., and Oppenheim, D., Eds.), pp 65-74.
Al-Khaldi, S. F., Coker, J., Shen, J. R., and Burnap, R. L. (2000) Characterization of site-directed mutants in manganese-stabilizing protein (MSP) of Synechocystis sp. PCC6803 unable to grow photoautotrophically in the absence of cytochrome c-550, Plant Mol Biol 43, 33-41.
Qian, M., Dao, L., Debus, R. J., and Burnap, R. L. (1999) Impact of mutations within the putative Ca2+-binding lumenal interhelical a-b loop of the photosystem II D1 protein on the kinetics of photoactivation and H2O -oxidation in Synechocystis sp. PCC6803, Biochemistry 38, 6070-6081.
Shen, J. R., Qian, M., Inoue, Y., and Burnap, R. L. (1998) Functional characterization of Synechocystis sp. PCC 6803 delta psbU and delta psbV mutants reveals important roles of cytochrome c-550 in cyanobacterial oxygen evolution, Biochemistry 37, 1551-1558.
Razeghifard, M. R., Wydrzynski, T., Pace, R. J., and Burnap, R. L. (1997) Yz. reduction kinetics in the absence of the manganese-stabilizing protein of photosystem II, Biochemistry 36, 14474-14478.
Qian, M., Al-Khaldi, S. F., Putnam-Evans, C., Bricker, T. M., and Burnap, R. L. (1997) Photoassembly of the photosystem II (Mn)4 cluster in site-directed mutants impaired in the binding of the manganese-stabilizing protein, Biochemistry 36, 15244-15252.
Putnam-Evans, C., Burnap, R., Wu, J., Whitmarsh, J., and Bricker, T. M. (1996) Site-directed mutagenesis of the CP 47 protein of photosystem II: alteration of conserved charged residues in the domain 364E-444R, Biochemistry 35, 4046-4053.
Meunier, P. C., Burnap, R. L., and Sherman, L. A. (1996) Improved 5-step modeling of the Photosystem II S-state mechanism in cyanobacteria, Photosynth Res 47, 61-76.
Burnap, R. L., Qian, M., and Pierce, C. (1996) The manganese stabilizing protein of photosystem II modifies the in vivo deactivation and photoactivation kinetics of the H2O oxidation complex in Synechocystis sp. PCC6803, Biochemistry 35, 874-882.
Shen, J. R., Burnap, R. L., and Inoue, Y. (1995) An independent role of cytochrome c-550 in cyanobacterial photosystem II as revealed by double-deletion mutagenesis of the psbO and psbV genes in Synechocystis sp. PCC 6803, Biochemistry 34, 12661-12668.
Meunier, P. C., Burnap, R. L., and Sherman, L. A. (1995) Interaction of the photosynthetic and respiratory electron transport chains producing slow apparent O2 releases under flashing light in Synechocystis sp. PCC 6803., Photosynth Res 45, 31-40.
Burnap, R. L., Qian, M., Shen, J. R., Inoue, Y., and Sherman, L. A. (1994) Role of disulfide linkage and putative intermolecular binding residues in the stability and binding of the extrinsic manganese-stabilizing protein to the photosystem II reaction center, Biochemistry 33, 13712-13718.
Burnap, R. L., Troyan, T., and Sherman, L. A. (1993) The highly abundant chlorophyll-protein complex of iron-deficient Synechococcus sp. PCC7942 (CP43') is encoded by the isiA gene, Plant Physiol 103, 893-902.
Burnap, R. L., Shen, J. R., Jursinic, P. A., Inoue, Y., and Sherman, L. A. (1992) Oxygen yield and thermoluminescence characteristics of a cyanobacterium lacking the manganese-stabilizing protein of photosystem II, Biochemistry 31, 7404-7410.
Burnap, R. L., and Sherman, L. A. (1991) Deletion mutagenesis in Synechocystis sp. PCC6803 indicates that the Mn-stabilizing protein of photosystem II is not essential for O2 evolution, Biochemistry 30, 440-446.
Burnap, R., Koike, H., Sotiropoulou, G., Sherman, L. A., and Inoue, Y. (1989) Oxygen-evolving membranes and particles from the transformable cyanobacterium Synechocystis sp. PCC6803, Photosynth Res 22, 121-130.
Burnap, R. L., and Trench, R. K. (1989) The biogenesis of the cyanellae of Cyanophora paradoxa. I. Polypeptide composition of the cyanellae, Proc R Soc Lond B Biol Sci 238, 53-72.
Burnap, R. L., and Trench, R. K. (1989) The biogenesis of the cyanellae of Cyanophora paradoxa. II. Pulse-labelling of cyanellar polypeptides in the presence of transcriptional and translational inhibitors, Proc R Soc Lond B Biol Sci 238, 73-87.
Burnap, R. L., and Trench, R. K. (1989) The biogenesis of the cyanellae of Cyanophora paradoxa. III. In vitro synthesis of cyanellar polypeptides using separated cytoplasmic and cyanellar RNA, Proc R Soc Lond B Biol Sci 238, 89-102.
Burnap, R. L., and Trench, R. K. (1984) Isolation and characterization of ribulose bisphosphate carboxylase from the cyanelles of Cyanophora paradoxa (Korschikoff), FEMS Microbiology Letters 21, 271-275.
Muscatine, L., Masuda, H., and Burnap, R. (1979) Ammonium Uptake by Symbiotic and Apo Symbiotic Reef Corals Madracis-Mirabilis, Bulletin of Marine Science 29 (4). 1979. 572-575.